Identification omega 3 and 6 on the positions sn-2 triacylglycerol Nile tilapia fish oil by hydrolysis using lipase from Mucor miehei

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M. Pandiangan, J. Kaban, B. Wirjosentono, J. Silalahi

2018 IOP Conference Series: Earth and Environmental Science Vol. 216 Issue 1 Conference paper Cited by 2 Quartile

Abstract

Omega 3 and 6 are unsaturated fatty acids needed by the body for cell formation and control of inflammation. In the molecules of omega 3 and 6 fats found in the position (stereospecific numbering) sn-1, 2 and 3. The position of fatty acids affects the digestion and absorption processes in the body. In the metabolism of fat in the body, fatty acids in the sn-2 position are not hydrolyzed so they can be absorbed properly. Therefore, it is necessary to identify the position of omega 3 and 6 fatty acids in fat molecules so that the potential of fish oils Nile tilapia as a source of omega 3 and 6 can be known. Fish oils Nile is extracted with the soxhletation method. The fatty acid composition was analyzed by gas chromatography (GC) which was previously esterified using BF 3 , for hydrolysis of fish oil using lipase enzyme. The results showed that the composition of unsaturated fatty acids was more than saturated fatty acids. Omega-3 and 6 fatty acids in the fat molecule are found more in the sn-2 than in the sn-1+sn-3 position. Seen from the composition and position fatty acids of Nile tilapia oil which contain omega 3 and 6 and there is a sn-2 position, so that more is absorbed in fat metabolism in the body. Thus fish oils Nile tilapia has the potential as a source of omega 3 and 6 from one of the freshwater fish that is widely consumed by the public. © 2018 Published under licence by IOP Publishing Ltd.

Affiliations

Postgraduate Student of Chemistry Science, Universitas Sumatera Utara, Medan, Indonesia; Department of Chemistry, Universitas Sumatera Utara, Medan, Indonesia; Department of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia; Agricultural Products Technology, Faculty of Agriculture, Universitas Katolik Santo Thomas, Medan, Indonesia